An unexpected role of semaphorin3A/neuropilin-1 signaling in lymphatic vessel maturation and valve formation
Identifieur interne : 004514 ( Main/Exploration ); précédent : 004513; suivant : 004515An unexpected role of semaphorin3A/neuropilin-1 signaling in lymphatic vessel maturation and valve formation
Auteurs : Giorgia Jurisic [Suisse] ; Hélène Maby-El Hajjami [Suisse] ; Sinem Karaman [Suisse] ; Alexandra M. Ochsenbein [Suisse] ; Annamari Alitalo [Suisse] ; Shoib S. Siddiqui [Suisse] ; Carlos Ochoa Pereira [Suisse] ; Tatiana V. Petrova [Suisse] ; Michael Detmar [Suisse]Source :
- Circulation research [ 0009-7330 ] ; 2012.
Abstract
Lymphatic vasculature plays important roles in tissue fluid homeostasis maintenance and in the pathology of human diseases. Yet, the molecular mechanisms that control lymphatic vessel maturation remain largely unknown.
We analyzed the gene expression profiles of
Lymphatic and blood vascular endothelial cells from mouse intestine were isolated using fluorescence-activated cell sorting and transcriptional profiling was performed. We found that the axonal guidance molecules Sema3A and Sema3D were highly expressed by lymphatic vessels. Importantly, we found that the semaphorin receptor Nrp-1 is expressed on the perivascular cells of the collecting lymphatic vessels. Treatment of mice
Together, these results reveal an unanticipated role of Sema3A/Nrp-1 signaling in the maturation of the lymphatic vascular network likely via regulating the perivascular cell coverage of the vessels thus affecting lymphatic vessel function and lymphatic valve development.
Url:
DOI: 10.1161/CIRCRESAHA.112.269399
PubMed: 22723300
PubMed Central: 3572231
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 003237
- to stream Pmc, to step Curation: 003236
- to stream Pmc, to step Checkpoint: 002B64
- to stream Ncbi, to step Merge: 004E80
- to stream Ncbi, to step Curation: 004E80
- to stream Ncbi, to step Checkpoint: 004E80
- to stream Main, to step Merge: 004536
- to stream Main, to step Curation: 004514
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">An unexpected role of semaphorin3A/neuropilin-1 signaling in lymphatic vessel maturation and valve formation</title><author><name sortKey="Jurisic, Giorgia" sort="Jurisic, Giorgia" uniqKey="Jurisic G" first="Giorgia" last="Jurisic">Giorgia Jurisic</name><affiliation wicri:level="1"><nlm:aff id="A1">Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich, 8093 Zurich, Switzerland</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea>Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich, 8093 Zurich</wicri:regionArea><wicri:noRegion>8093 Zurich</wicri:noRegion></affiliation></author><author><name sortKey="Hajjami, Helene Maby El" sort="Hajjami, Helene Maby El" uniqKey="Hajjami H" first="Hélène Maby-El" last="Hajjami">Hélène Maby-El Hajjami</name><affiliation wicri:level="1"><nlm:aff id="A2">Division of Experimental Oncology CePO, CHUV and University of Lausanne, 1066 Epalinges, Switzerland</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea>Division of Experimental Oncology CePO, CHUV and University of Lausanne, 1066 Epalinges</wicri:regionArea><wicri:noRegion>1066 Epalinges</wicri:noRegion></affiliation></author><author><name sortKey="Karaman, Sinem" sort="Karaman, Sinem" uniqKey="Karaman S" first="Sinem" last="Karaman">Sinem Karaman</name><affiliation wicri:level="1"><nlm:aff id="A1">Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich, 8093 Zurich, Switzerland</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea>Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich, 8093 Zurich</wicri:regionArea><wicri:noRegion>8093 Zurich</wicri:noRegion></affiliation></author><author><name sortKey="Ochsenbein, Alexandra M" sort="Ochsenbein, Alexandra M" uniqKey="Ochsenbein A" first="Alexandra M." last="Ochsenbein">Alexandra M. Ochsenbein</name><affiliation wicri:level="1"><nlm:aff id="A1">Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich, 8093 Zurich, Switzerland</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea>Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich, 8093 Zurich</wicri:regionArea><wicri:noRegion>8093 Zurich</wicri:noRegion></affiliation></author><author><name sortKey="Alitalo, Annamari" sort="Alitalo, Annamari" uniqKey="Alitalo A" first="Annamari" last="Alitalo">Annamari Alitalo</name><affiliation wicri:level="1"><nlm:aff id="A1">Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich, 8093 Zurich, Switzerland</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea>Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich, 8093 Zurich</wicri:regionArea><wicri:noRegion>8093 Zurich</wicri:noRegion></affiliation></author><author><name sortKey="Siddiqui, Shoib S" sort="Siddiqui, Shoib S" uniqKey="Siddiqui S" first="Shoib S." last="Siddiqui">Shoib S. Siddiqui</name><affiliation wicri:level="1"><nlm:aff id="A1">Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich, 8093 Zurich, Switzerland</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea>Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich, 8093 Zurich</wicri:regionArea><wicri:noRegion>8093 Zurich</wicri:noRegion></affiliation></author><author><name sortKey="Pereira, Carlos Ochoa" sort="Pereira, Carlos Ochoa" uniqKey="Pereira C" first="Carlos Ochoa" last="Pereira">Carlos Ochoa Pereira</name><affiliation wicri:level="1"><nlm:aff id="A1">Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich, 8093 Zurich, Switzerland</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea>Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich, 8093 Zurich</wicri:regionArea><wicri:noRegion>8093 Zurich</wicri:noRegion></affiliation></author><author><name sortKey="Petrova, Tatiana V" sort="Petrova, Tatiana V" uniqKey="Petrova T" first="Tatiana V." last="Petrova">Tatiana V. Petrova</name><affiliation wicri:level="1"><nlm:aff id="A2">Division of Experimental Oncology CePO, CHUV and University of Lausanne, 1066 Epalinges, Switzerland</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea>Division of Experimental Oncology CePO, CHUV and University of Lausanne, 1066 Epalinges</wicri:regionArea><wicri:noRegion>1066 Epalinges</wicri:noRegion></affiliation></author><author><name sortKey="Detmar, Michael" sort="Detmar, Michael" uniqKey="Detmar M" first="Michael" last="Detmar">Michael Detmar</name><affiliation wicri:level="1"><nlm:aff id="A1">Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich, 8093 Zurich, Switzerland</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea>Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich, 8093 Zurich</wicri:regionArea><wicri:noRegion>8093 Zurich</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">22723300</idno><idno type="pmc">3572231</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3572231</idno><idno type="RBID">PMC:3572231</idno><idno type="doi">10.1161/CIRCRESAHA.112.269399</idno><date when="2012">2012</date><idno type="wicri:Area/Pmc/Corpus">003237</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">003237</idno><idno type="wicri:Area/Pmc/Curation">003236</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">003236</idno><idno type="wicri:Area/Pmc/Checkpoint">002B64</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">002B64</idno><idno type="wicri:Area/Ncbi/Merge">004E80</idno><idno type="wicri:Area/Ncbi/Curation">004E80</idno><idno type="wicri:Area/Ncbi/Checkpoint">004E80</idno><idno type="wicri:doubleKey">0009-7330:2012:Jurisic G:an:unexpected:role</idno><idno type="wicri:Area/Main/Merge">004536</idno><idno type="wicri:Area/Main/Curation">004514</idno><idno type="wicri:Area/Main/Exploration">004514</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">An unexpected role of semaphorin3A/neuropilin-1 signaling in lymphatic vessel maturation and valve formation</title><author><name sortKey="Jurisic, Giorgia" sort="Jurisic, Giorgia" uniqKey="Jurisic G" first="Giorgia" last="Jurisic">Giorgia Jurisic</name><affiliation wicri:level="1"><nlm:aff id="A1">Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich, 8093 Zurich, Switzerland</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea>Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich, 8093 Zurich</wicri:regionArea><wicri:noRegion>8093 Zurich</wicri:noRegion></affiliation></author><author><name sortKey="Hajjami, Helene Maby El" sort="Hajjami, Helene Maby El" uniqKey="Hajjami H" first="Hélène Maby-El" last="Hajjami">Hélène Maby-El Hajjami</name><affiliation wicri:level="1"><nlm:aff id="A2">Division of Experimental Oncology CePO, CHUV and University of Lausanne, 1066 Epalinges, Switzerland</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea>Division of Experimental Oncology CePO, CHUV and University of Lausanne, 1066 Epalinges</wicri:regionArea><wicri:noRegion>1066 Epalinges</wicri:noRegion></affiliation></author><author><name sortKey="Karaman, Sinem" sort="Karaman, Sinem" uniqKey="Karaman S" first="Sinem" last="Karaman">Sinem Karaman</name><affiliation wicri:level="1"><nlm:aff id="A1">Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich, 8093 Zurich, Switzerland</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea>Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich, 8093 Zurich</wicri:regionArea><wicri:noRegion>8093 Zurich</wicri:noRegion></affiliation></author><author><name sortKey="Ochsenbein, Alexandra M" sort="Ochsenbein, Alexandra M" uniqKey="Ochsenbein A" first="Alexandra M." last="Ochsenbein">Alexandra M. Ochsenbein</name><affiliation wicri:level="1"><nlm:aff id="A1">Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich, 8093 Zurich, Switzerland</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea>Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich, 8093 Zurich</wicri:regionArea><wicri:noRegion>8093 Zurich</wicri:noRegion></affiliation></author><author><name sortKey="Alitalo, Annamari" sort="Alitalo, Annamari" uniqKey="Alitalo A" first="Annamari" last="Alitalo">Annamari Alitalo</name><affiliation wicri:level="1"><nlm:aff id="A1">Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich, 8093 Zurich, Switzerland</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea>Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich, 8093 Zurich</wicri:regionArea><wicri:noRegion>8093 Zurich</wicri:noRegion></affiliation></author><author><name sortKey="Siddiqui, Shoib S" sort="Siddiqui, Shoib S" uniqKey="Siddiqui S" first="Shoib S." last="Siddiqui">Shoib S. Siddiqui</name><affiliation wicri:level="1"><nlm:aff id="A1">Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich, 8093 Zurich, Switzerland</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea>Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich, 8093 Zurich</wicri:regionArea><wicri:noRegion>8093 Zurich</wicri:noRegion></affiliation></author><author><name sortKey="Pereira, Carlos Ochoa" sort="Pereira, Carlos Ochoa" uniqKey="Pereira C" first="Carlos Ochoa" last="Pereira">Carlos Ochoa Pereira</name><affiliation wicri:level="1"><nlm:aff id="A1">Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich, 8093 Zurich, Switzerland</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea>Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich, 8093 Zurich</wicri:regionArea><wicri:noRegion>8093 Zurich</wicri:noRegion></affiliation></author><author><name sortKey="Petrova, Tatiana V" sort="Petrova, Tatiana V" uniqKey="Petrova T" first="Tatiana V." last="Petrova">Tatiana V. Petrova</name><affiliation wicri:level="1"><nlm:aff id="A2">Division of Experimental Oncology CePO, CHUV and University of Lausanne, 1066 Epalinges, Switzerland</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea>Division of Experimental Oncology CePO, CHUV and University of Lausanne, 1066 Epalinges</wicri:regionArea><wicri:noRegion>1066 Epalinges</wicri:noRegion></affiliation></author><author><name sortKey="Detmar, Michael" sort="Detmar, Michael" uniqKey="Detmar M" first="Michael" last="Detmar">Michael Detmar</name><affiliation wicri:level="1"><nlm:aff id="A1">Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich, 8093 Zurich, Switzerland</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea>Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich, 8093 Zurich</wicri:regionArea><wicri:noRegion>8093 Zurich</wicri:noRegion></affiliation></author></analytic><series><title level="j">Circulation research</title><idno type="ISSN">0009-7330</idno><idno type="eISSN">1524-4571</idno><imprint><date when="2012">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec id="S1"><title>Rationale</title><p id="P1">Lymphatic vasculature plays important roles in tissue fluid homeostasis maintenance and in the pathology of human diseases. Yet, the molecular mechanisms that control lymphatic vessel maturation remain largely unknown.</p></sec><sec id="S2"><title>Objective</title><p id="P2">We analyzed the gene expression profiles of <italic>ex vivo</italic> isolated lymphatic endothelial cells to identify novel lymphatic vessel expressed genes and we investigated the role of Sema3A and neuropilin-1 (Nrp-1) in lymphatic vessel maturation and function.</p></sec><sec id="S3"><title>Methods and results</title><p id="P3">Lymphatic and blood vascular endothelial cells from mouse intestine were isolated using fluorescence-activated cell sorting and transcriptional profiling was performed. We found that the axonal guidance molecules Sema3A and Sema3D were highly expressed by lymphatic vessels. Importantly, we found that the semaphorin receptor Nrp-1 is expressed on the perivascular cells of the collecting lymphatic vessels. Treatment of mice <italic>in utero</italic> (E12.5-E16.5) with an antibody that blocks Sema3A binding to Nrp-1, but not with an antibody that blocks VEGF-A binding to Nrp-1, resulted in a complex phenotype of impaired lymphatic vessel function, enhanced perivascular cell coverage and abnormal lymphatic vessel and valve morphology.</p></sec><sec id="S4"><title>Conclusions</title><p id="P4">Together, these results reveal an unanticipated role of Sema3A/Nrp-1 signaling in the maturation of the lymphatic vascular network likely via regulating the perivascular cell coverage of the vessels thus affecting lymphatic vessel function and lymphatic valve development.</p></sec></div></front></TEI><affiliations><list><country><li>Suisse</li></country></list><tree><country name="Suisse"><noRegion><name sortKey="Jurisic, Giorgia" sort="Jurisic, Giorgia" uniqKey="Jurisic G" first="Giorgia" last="Jurisic">Giorgia Jurisic</name></noRegion><name sortKey="Alitalo, Annamari" sort="Alitalo, Annamari" uniqKey="Alitalo A" first="Annamari" last="Alitalo">Annamari Alitalo</name><name sortKey="Detmar, Michael" sort="Detmar, Michael" uniqKey="Detmar M" first="Michael" last="Detmar">Michael Detmar</name><name sortKey="Hajjami, Helene Maby El" sort="Hajjami, Helene Maby El" uniqKey="Hajjami H" first="Hélène Maby-El" last="Hajjami">Hélène Maby-El Hajjami</name><name sortKey="Karaman, Sinem" sort="Karaman, Sinem" uniqKey="Karaman S" first="Sinem" last="Karaman">Sinem Karaman</name><name sortKey="Ochsenbein, Alexandra M" sort="Ochsenbein, Alexandra M" uniqKey="Ochsenbein A" first="Alexandra M." last="Ochsenbein">Alexandra M. Ochsenbein</name><name sortKey="Pereira, Carlos Ochoa" sort="Pereira, Carlos Ochoa" uniqKey="Pereira C" first="Carlos Ochoa" last="Pereira">Carlos Ochoa Pereira</name><name sortKey="Petrova, Tatiana V" sort="Petrova, Tatiana V" uniqKey="Petrova T" first="Tatiana V." last="Petrova">Tatiana V. Petrova</name><name sortKey="Siddiqui, Shoib S" sort="Siddiqui, Shoib S" uniqKey="Siddiqui S" first="Shoib S." last="Siddiqui">Shoib S. Siddiqui</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004514 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 004514 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= LymphedemaV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= PMC:3572231
|texte= An unexpected role of semaphorin3A/neuropilin-1 signaling in lymphatic vessel maturation and valve formation
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:22723300" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a LymphedemaV1
| This area was generated with Dilib version V0.6.31. |  |